Yohana Theresia Maria Astuti Primary Author Kumala Dewi Additional Author Santosa Additional Author A. Adi Prawoto Additional Author mixed material bibliography yogyakarta UPN VETERAN YOGYAKARTA 2013 monographic ICGAI, Yogyakarta, INDONESIA, November 12-14, 2013

Text

Proceedings International Conference on Green Agro An experiment was carried out to determine gibberellic acid synthesis in the developing cacao seeds. Two cacao clones namely KW163 and KW165 were used. Several plants for each clone were selected and hand pollination was conducted before anthesis. Samples of healthy seeds were taken at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 weeks following hand pollination. Gibberellic acid content was determined by HPLC (High Performance Liquid Chromatography). Molecular study was carried out to know expression of TcGA20ox like gene on seeds aged seven weeks. The results showed that, in seeds aged 1-10 weeks, the giberellic acid content in developing seeds of clone KW163 fluctuated from about 1068.10 ng.g -1 fw at week 1 to 181.92 ng.g -1 fw at week 10 and the giberellin content in developing seeds of clone KW165 fluctuated from about 1762.49 ng.g -1 fw at week 1 to 714.54 ng.g -1 fw at week 10. TcGA20ox like gene that could be found in seeds indicated that there was gibberellic acid synthesis in the developing seeds. Keywords: Cocoa seed, synthesis, gibberellic acid, TcGA20ox like gene NONE Institutional Repository Institut Pertanian STIPER Yogyakarta ISBN: 978-979-18768-3-4 ART.DOS.065 My Library (ARTIKEL) GIBBERELLIC ACID SYNTHESIS IN THE DEVELOPING SEEDS OF COCOA 18467 2022-03-21 12:05:36 2022-03-21 12:10:49 machine generated